Module 1: BIOINFORMATICS

Bioinformatics: Introduction, Applications and Scope. Tools for finding the similarity in sequence databases and multiple sequence alignment: FASTA, BLAST (BLASTP, BLASTN, BLASTX, TBLASTX, TBLASTN), ClustalW. Overview of Biological Databases: Primary & Secondary Databases, Protein Structure, and Family Databases (PDB, CATH, SCOP, Pfam, PIR, PROSITRE, Swiss-Prot), Sequence Databases (GenBank, EMBL, DDBJ), Phylogenetic Analysis: A Comprehensive Understanding. 

 

Module 2: MICROBIOLOGY

Introduction to Good Laboratory Practices and General Safety Instructions, Instrumentation: Working with Autoclave, Laminar Air  Flow System, Incubator/ Shaker, Hot Air Oven, Various Media (Solid/Liquid) preparation and Its Sterilization, Isolation and Culturing of microbes from Soil Samples, Screening of Microbes(Staining Techniques): Gram Staining, Endospore Staining, Antibiotics Sensitivity Test, Isolation and identification of enzyme producing microbe, Isolation of antibiotic resistance (MDR) microbe from soil sample.

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Module 3: MOLECULAR BIOLOGY

Good Laboratory Practices and General Safety Instructions, Basics of Calculations; Buffers and Reagent Preparation, Culturing of Microbes(E. coli DH5α Strain for Genomic DNA Isolation), Isolation and Purification of Prokaryotic (Bacterial) Genomic DNA, Isolation & Purification of Genomic DNA from Plants, Agarose Gel Electrophoresis of Isolated  Genomic and plasmid  DNA, Quantitative analysis of Nucleic Acids using Nanodrop/ Qubit 2.0, Primer Designing and Optimization of PCR

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Module 4: BIOCHEMISTRY

The fundamentals of biochemistry, GLP (good laboratory practises),  The concept of elementary calculations, Using forward and reverse pipetting techniques to handle micropipettes, Fundamental, SOP, Using and handling of instruments (pH metre, colorimeter, spectrophotometer, weighing balance), Protein Concentration Determination Using the Bradford Method .  Peptide bond detection using the biuretic reaction, Calculating Sugar's reducing and non-reducing amounts, Standard graph preparation, data interpretation, and the DNS reagent's principle are all included in the quantitative measurement of carbohydrates using the DNS method.

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Module 5: MEDICAL MICROBIOLOGY

Introduction to Good Laboratory Practices and General Safety Instructions, Instrumentation: Working with Autoclave, Laminar Air Flow System, Incubator/ Shaker, Hot Air Oven, Microscope and Other Instruments, Various Media (Solid/Liquid) preparation and Its Sterilization, Isolation and Screening of Microorganisms from clinical samples, Identification of Bacteria of Clinical Importance by various Staining Method, Urine Sample Analysis & Antibiotic Sensitivity Test, Enzymatic Assay: Catalase activity Test, Oxidase activity Test, Blood Microscopy

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Module 6: IMMUNOLOGY

Introduction to Immunology, basic instrument handling, concept of basic calculation, solution preparation, Micropipette handling (forward or reverse pipetting techniques), Components of blood, Basic concept of antigen and antibody, Bleeding time calculation, Clotting time estimation, To Perform Blood Grouping Test, To detect the Presence of Salmonella sp. by Qualitative Slide Agglutination Test (Widal Test),Isolation of Serum and Plasma from Blood, To Perform VDRL Test, ELISA Test, To Perform Radial Immunodiffusion Test

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Module 7: FOOD BIOTECHNOLOGY

Introduction to the food Microbiology Laboratory practice and Equipments, Lab Safety& Ethics, Preparation of chemical & Reagents used in food tech laboratory, Sterilization techniques for lab equipments and culture media, Isolation of microbes from various food items, Enumeration and isolation of E. Coli from food sample, Quality control of Milk and Milk products, Determination of pH and acidity of food sample, To estimate the moisture content of food items, Determination of food adulteration in various commercial food items, Antioxidant analysis of Green tea products

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Module 8: FORENSIC SCIENCE

Cleaning & Sterilization of glasswares, Preparation of chemical& Reagents used in forensic science laboratory, Safety Information, Maintenance of Sterility & Do’s & Don’ts in the Forensic Lab, To prepare slides of scale patterns of human hair, To determine blood group from fresh blood and dried blood stains, To identify blood and saliva stains, To prepare gel plates for electrophoresis, Quantification of DNA using Nano drop method

 

Module 9: ENVIRONMENTAL BIOLOGY

Introduction to Environmental Biology, Scope of Environmental Biology, Good laboratory practices (GLP), SOP and application of instrument used in environmental biology laboratory, Concept of basic calculation (Percentage, molarity and normality), overview of environmental components( Air, water, soil), Principle and application of pH meter, types of buffers, pH and moisture content determination of soil/water sample, salinity test of soil/water sample, calculation of total dissolved solids (TDS) in water, TDS determination of water, Analysis of Total suspended solids (TSS) in waste/soil, Determine the free chloride in water, Analysis of Carbonate ions.

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Module 10: ENZYMOLOGY

Introduction and applications of enzymology, Types of enzymes, Good laboratory practices (GLP), Instrument handling, Concept of basic calculation (Percentage, molarity and normality), Effect of different parameters on enzyme production, Uses of industrial enzyme in different industries, Screening of enzyme producing bacteria from soil/ water, Screening of Amylase enzyme from fungus and bacteria, Amylase activity assay, Protease Enzyme activity assay, Analysis of Catalase, Urease, Identification of citrate producing bacteria.

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Module 11: PHARMACOLOGY

Introduction of pharmaceutical chemistry, General and safety rules for working in pharma laboratory, Instrument handling, Concept of different calculations, Buffer and solution preparation, Types of media, Sterilization of glassware, preparation and sterilization of media, Detection of antimicrobial activity of antibiotics, Presence of nitrogen in sample, Presence of sulphur, Presence of halogens group, Presence of sugar, presence of non-reducing sugar, Qualitative analysis of different drugs and antibiotics, Basics of limit test in Pharma, Limit test of chloride.

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Module 12: PROTEOMICS

Introduction, Applications and Scope of proteomics, Good laboratory practices (GLP) and General Safety Instructions, The concept of elementary calculations, Buffers and Reagent Preparation, Using forward and reverse pipetting techniques to handle micropipettes, Instrument handling (pH meter, colorimeter, spectrophotometer, weighing balance), Protein isolation from Plant sample, estimation of protein using Bradford Method/ Biuret Method/ Lowry’s Method, Protein separation by SDS – PAGE, Detailed overview of Mass Spectrometry.

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Module 13: HEMATOLOGY

Introduction of hematology laboratery, Instrument handling, concept of basic calculation, solution preparation, Microscope handling, Familiarization and working of routine hematology laboratory, Good laboratory practices (GLP) and General Safety Instructions, Instrument handling, Hemoglobin  estimation in whole blood, Determine of RBC & WBC present in one cubic mm volume of  blood, Microscopic examination of  blood cells, Agglutination analysis on human blood, Bleeding time calculation, Clotting time estimation, Separation of Serum From human blood.

 

Module 14: BLOOD BIOCHEMISTRY

Introduction of biochemistry lab, Instrument handling, concept of basic calculation, Preparation of buffer solution and measurement of pH, Good laboratory practices (GLP) and General Safety Instructions, Determination of blood sugar, Determination of total cholesterol, Alkaline phosphatase enzyme assay from serum sample, Determination of Blood urea from serum sample.

 

Module 15: CLINICAL BIOTECHNOLOGY

Introduction of clinical laboratory, Instrument handling, concept of basic calculation, Preparation of buffer solution and measurement of pH, Good laboratory practices (GLP) and General Safety Instructions, Microscope handling, Qualitative analysis of urine for abnormal constituents, Urine microscopy, Urine culture, cell counting in urine sediment, Antimicrobial assay.

 

Module 16: CANCER BIOLOGY/ ANIMAL CELL CULTURE

Introduction of animal cell culture laboratory, Instrument handling, concept of basic calculation, Preparation of buffer solution and measurement of pH, Good laboratory practices (GLP) and General Safety Instructions, Inverted microscope handling, introduction  of different media used in cell culture, Media preparation, Media sterilization using syringe filters, cell seeding in T-25  flask, cell confluency, Trypsinization of cells, trypan blue exclusion assay.

 

Module 17: NANO BIOTECHNOLOGY

Introduction of biotech laboratory, Instrument handling, concept of basic calculation, Preparation of buffer solution and measurement of pH, Good laboratory practices (GLP) and General Safety Instructions, Spectrophotometer handling, introduction  of different types of Nano particles and there size, plant extract preparation, formation of silver nanoparticles, formation of copper nanoparticles, collection of nanoparticles, characterization of nano-particles using spectrophotometer, antimicrobial activity of nanoparticles.    

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Module 18: PLANT METABOLOMICS

Introduction of biotech laboratory, Instrument handling, concept of basic calculation, Preparation of buffer solution and measurement of pH, Good laboratory practices (GLP) and General Safety Instructions, Spectrophotometer handling, introduction of different phytochemicals found in plants, Plant extract preparation, estimation of different phytochemicals, antimicrobial activity of plant extract, Antioxidant activity (DPPH Assay) of plant extract, IC-50 value calculation.