Molecular Biology & rDNA Technology

Embark on an enriching journey into the intricate world of molecular biology with our hands-on training program. Delve into the fundamentals of molecular biology through interactive laboratory sessions, where you'll learn essential techniques such as DNA extraction, PCR amplification, gel electrophoresis, and DNA sequencing. Collaborate with knowledgeable instructors who will guide you in experimental design, data analysis, and interpretation, providing a solid foundation for molecular research and diagnostics.

Develop crucial laboratory skills, critical thinking abilities, and attention to detail, equipping you to pursue careers in research, healthcare, biotechnology, and beyond, with a deep understanding of molecular biology principles and applications.

15 Days/2 Weeks Program:

• Good Laboratory Practices and General Safety Instructions

• Basics of Calculations; Buffers and Reagent Preparation

• Sterilization Techniques: Dry, Wet and Chemical Sterilization

• Preparation of Luria-Bertani Media and it’s Sterilization

• Culturing of Microbes(E. coli DH5αStrain for Plasmid/ Genomic DNA Isolation)

• Isolation and Purification of Prokaryotic (Bacterial) Genomic DNA

• Agarose Gel Electrophoresis of Genomic DNA

• Isolation & Purification of Genomic DNA from Plants

• Agarose Gel Electrophoresis of Isolated Plant Genomic DNA

• Isolation & Purification of Plasmid DNA

• Determination of Purity and Quantification of Plasmid DNA

• Agarose Gel Electrophoresis of Isolated Plasmid DNA

• Quantitative analysis of Nucleic Acids using DPA reagent

• Qualitative (A260/280) & Quantitative analysis of Nucleic Acids using Nanodrop/Qubit 2.0

• Primer Designing and Optimization of PCR

• Polymerase Chain Reaction(PCR)

• Gel Electrophoresis of PCR Products with Molecular Markers

30 Days/4 Weeks Program:

• Working in Molecular Biology Laboratory

• Good Laboratory Practices and General Safety Instructions

• Basics of Calculations; Buffers and Reagent Preparation

• Sterilization Techniques: Dry, Wet and Chemical Sterilization

• Isolation and Purification of Prokaryotic (Bacterial) Genomic DNA

• Agarose Gel Electrophoresis of Genomic DNA

• Preparation of Luria Bertani Media and it’s Sterilization

• Culturing of Microbes(E. coli DH5α Strain for Plasmid DNA Isolation)

• Isolation & Purification of Plasmid DNA from E. coli DH5α Strain

• Agarose Gel Electrophoresis of Isolated Plasmid DNA

• Determination of Purity and Quantification of Isolated Plasmid DNA

• Isolation & Purification of Genomic DNA from Plants

• Agarose Gel Electrophoresis of Isolated Plant Genomic DNA

• RNA Isolation from Plant Sources

• Denaturing Agarose Gel Electrophoresis of Isolated RNA

• Northern Blotting Technique (RNA from Gel to membrane)

• Purification & Quantification of RNA

• Quantitative analysis of Nucleic Acids using DPA reagent

• Qualitative (A260/280) & Quantitative analysis of Nucleic Acids using Nanodrop/Qubit2.0

• Primer Designing and Optimization of PCR

• Polymerase Chain Reaction(PCR)

• Gel Electrophoresis of PCR Products with Molecular Markers

• Restriction Digestion of DNA

• Electrophoresis of Digested DNA Product

• Southern Blotting Technique(Transfer of DNA to Membrane)

• Competent Cell Preparation of E. coli

• cDNA Preparation and Cloning

• Cloning of cDNA into Digested Plasmid (cDNA Ligation)

• Transformation of Ligated Plasmid into Competent Cells

• Screening of the Transformed Cells (Blue-White selection)

• Colony PCR for Screening of Transformed Cells