Advance rDNA tech & Animal Cell Culture
This module include hands-on laboratory sessions where participants learn cell culture techniques, such as subculturing, passaging, and cryopreservation of cancer cells. They also learn about quality control measures, cell viability assays, and monitoring cell growth and behavior. Additionally, participants receive training on experimental techniques and assays used in cancer research, such as drug screening, genetic manipulation, & molecular analysis of cancer cells.
Cancer cell culture and molecular biology techniques empower researchers to unravel the complexities of cancer, facilitating the development of novel treatments and improved understanding of the disease.
30 Days/ 4 Weeks Program:
Safety Information & Maintenance of Sterility
Do’s & Don’ts in the Tissue Culture Lab
Exposure to Facility equipment
Preparation of medium and required Solutions
Cell Lines revival (Recovery)
Animal Cell Culture and maintenance of human cancer cell lines
Sub culturing (passaging) & freezing
Freezing & thawing of human cancer cell lines
RNA extraction from human cancer cell lines
cDNA synthesis
Reverse Transcription PCR (RT-PCR)
Inverted Microscopy
Induction of proliferation & differentiation in human cancer cell lines
Protein extraction from human cancer cell lines
SDS-PAGE and coomassie blue staining
MTT Assay for proliferation/cytotoxicity
Restriction digestion
Ligation
DNA gel electrophoresis
DNA gel extraction
Preparation of competent cells
Transformation and plating on LBA plates
Plasmid DNA isolation
Genomic DNA isolation
Polymerase Chain Reaction (PCR)
Protein & DNA sequence analysis
Primer Designing & Promoter analysis
